A&P II - LABORATORY PRACTICAL EXAM 1
ENDOCRINE TISSUE MICROSCOPE SLIDES:
Pituitary gland (hypophysis)
Anterior lobe (adenohypophysis)
Posterior lobe (neurohypophysis)
Thyroid gland Testis
Follicle Seminiferous tubule(s)
Colloid Interstitial cell(s)
Parafollicular cell(s)
Ovary
Parathyroid gland Follicle
Antrum
Pancreas Egg (ovum)
Acini(us) Corona radiata
Islet (of Langerhans)
Adrenal gland
Capsule
Adrenal cortex
Zona glomerulosa
Zona fasciculata
Zona reticularis
Adrenal medulla
BLOOD SLIDES
Erythrocytes (red blood cells) Thrombocytes (platelets)
Leucocytes: neutrophil Mast cells (in areolar ct)
Eosinophil
Basophil Sickled RBC (from sickle-
lymphocyte cell anemia slides)
monocyte
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The following observational criteria may assist in identifying specific
tissues or cells:
Adenohypophysis: highly cellular mass adjacent to neurohypophysis
Will not be able to distinguish acidophils,
basophils, or chromophobes
Neurohypophysis: consists of neuron fibers and relatively few cells
(pituicytes)
Adjacent to highly cellular adenohypophysis
Thyroid: Lobules consist of many follicles (spherical arrays of cells–
1 layer thick – in circular x.s. shape. Cells vary from
squamous-like to cuboidal, depending on level of activity
Central portion of follicle contains colloid – a viscous
thyroglobin-rich fluid. Between or adjacent to the follicles
are parafollicular, or C cells
Also visible may be blood vessels
Parathyroid: Highly cellular mass of more-or-less homogeneous cells
adjacent to the thyroid (probably cannot distinguish between
oxyphil and chief cells – most are chief)
Pancreas: Most of slide consists of exocrine acini (us) surrounding
isolated clusters – islets of Langerhans. Can not
distinguish alpha and beta cells.
Also visible – blood vessels and exocrine ducts
Adrenal gland: Cortex – consists of three zones covered by outer capsule of
fibrous connective tissue
Zona glomerulosa – outermost layer – clusters of
cells
Zona fascilulata - middle layer – strings of cells
Zona reticularis – inner layer – network of cells
Medulla – center of gland – consists of chromaffin
cells with may spaces or gaps
Ovary: ovarian follicle – spherical array of cells, similar
in overall appearance to thyroid follicles
– circular in x.s.
houses an immature egg surrounded by a layer of cells
(corona radiata) and attached to follicle
wall.
fluid-filled center (antrum)
Testis: Slide shows many x.s. views of seminiferous tubules;
Lumen may contain immature sperms
Clusters of cells between tubules are
interstitial cells
Erythrocytes (RBCs): small pink cells with lighter centers – hundreds in
view
Thrombocytes: (platelets) – tiny fragments of cells much smaller than RBCs
and stained purplish
Neutrophils (WBCs): multilobed nucleus – granules stail light lilac and
may be difficult to see
Eosinophils: WBCs – bilobed nucleus – reddish granules (may vary
from red, red-orange, red-brown, red-purple)
Basophils: WBCs – bilobed nucleus; cell is characterized by blue
or blue-purple granules. Granules are so
numerous they usually obscure the nucleus
Lymphocytes: agranular – large purple-stained non-lobed nucleus.
Cytoplasm usually light blue
Monocytes: agranular – large purple non-lobed nucleus, although
nucleus usually kidney-shaped. Cytoplasm
stained blue-gray
Mast cells: Connective tissue cell related to basophils
Seen in areolar ct and characterized by abundance of
granules
